Since ancient times, plants have been t h e main source of natural medicines and many therapeutic agents. Terminalia arjuna (Arjun) bark powder is widely used in cardiovascular and liver diseases with blood coagulating properties. Its methanolic extract was prepared by Soxhlet apparatus for 6 h. Organic constituents were separated by column chromatography and eluted with different solvents of increasing polarity whereby three compounds with Rf values 0.79, 0.63 and 0.72 were separated by thin layer chromatography (TLC). After purification, the compounds were analyzed for C, H and N contents and identified by infrared (ir) and nuclear magnetic resonance (NMR) spectroscopic studies. Finally, the compounds were confirmed by fragmentation pattern obtained by gas chromatographymass spectrometric (GC-MS) studies. The compounds separated and identified were 1-phenylazo 2-naphthalenol (PAN), 2-isopropyl naphtho [2,3-β] furan-4, 9-dione (INFD) and tartaric acid (TA). It is proposed that PAN and TA may act as ligands to bind with nutrient elements making them bioavailable to the body system.